![]() Under sultable conditions, two single nucleic-acid chains form a hybrid molecule to an extent that is largely dependent on the degree of their nucleotide complementarity. 1989 Nov 189(2):175–184.The detection and identification of specific nucleic acid sequences is routine in molecular biology research The principles that govern molecular annealing (or molecular hybridization) are used in many apphcatlons to the study of nucleic acids. Molecular epidemiology of Pseudomonas aeruginosa-urinary tract infections in paraplegic patients. Worlitzsch D, Wolz C, Botzenhart K, Hansis M, Burgdörfer H, Ogle JW, Döring G.Pseudomonas aeruginosa cross-colonization and persistence in patients with cystic fibrosis. Wolz C, Kiosz G, Ogle JW, Vasil ML, Schaad U, Botzenhart K, Döring G.Nosocomial acquisition of Pseudomonas aeruginosa by cystic fibrosis patients. Tümmler B, Koopmann U, Grothues D, Weissbrodt H, Steinkamp G, von der Hardt H.Use of a pilin gene probe to study molecular epidemiology of Pseudomonas aeruginosa. Speert DP, Campbell ME, Farmer SW, Volpel K, Joffe AM, Paranchych W. ![]() Detection of specific sequences among DNA fragments separated by gel electrophoresis. Biotinylated DNA probes for exotoxin A and pilin genes in the differentiation of Pseudomonas aeruginosa strains. Person-to-person transmission of Pseudomonas cepacia between patients with cystic fibrosis. LiPuma JJ, Dasen SE, Nielson DW, Stern RC, Stull TL.Interactions of Pseudomonas aeruginosa proteases with the cells of the immune system. Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains. Hancock RE, Mutharia LM, Chan L, Darveau RP, Speert DP, Pier GB.Genome fingerprinting of Pseudomonas aeruginosa indicates colonization of cystic fibrosis siblings with closely related strains. Grothues D, Koopmann U, von der Hardt H, Tümmler B.Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (993K), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. The patterns of hybridization to the elastase and algD gene probes were highly conserved in all isolates, therefore, these DNA fragments are not useful in discriminating strains, in contrast to the exotoxin A gene probe. aeruginosa were used in Southern hybridization analysis. DNA fragments from the alginate biosynthesis gene complex, the exotoxin A gene, and the elastase gene of P. aeruginosa DNAs from cystic fibrosis isolates however, sequential isolates obtained from individual patients showed very little variation over an 8-year period. There was marked diversity of restriction enzyme analysis patterns among P. aeruginosa isolates from patients with cystic fibrosis were examined by restriction enzyme analysis and Southern hybridization. Conventional typing schemes for Pseudomonas aeruginosa may not discriminate strains.
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